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Cloning of Putative Repetitive Domains of the ...

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Title Information
Cloning of Putative Repetitive Domains of the Glycoprotein from the Sticky Aqueous Coat of the Spider, Araneus gemmoides – A Complementary Study Using DNA Libraries

Name:Personal
Bayarmagnai, Battuya Role :Text(marcrelator)
creator

Name:Personal
Molecular Biology; Dr. Randy Lewis Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-23

Language:Text
eng

Physical Description

born digital

abstract
Orb- weaving spiders produce the largest variety of silk types and make an extensive use of them. Sticky spiral flagelliform silk is covered with an aqueous coat which is essential in capturing prey. It has been found that the coat consists of high concentrations of water-soluble organic compounds, high molecular weight compounds such as glycoprotein and low concentrations of different inorganic salts. The glycoprotein has been identified as the only compound that has a sufficiently high molecular weight to act as an actual glue. Previously, cDNA library of the orb-weaving spider Araneus gemmoides was screened for the gene-specific fragments. A repetitive part that consisted of about 500 base pairs was cloned. In this study, cloning of the glycoprotein gene of A. gemmoides has been continued. The DNA library has been screened for the glycoprotein gene with a DNA probe specific to the repetitive part of the gene. Collected clones were analyzed which revealed a 900 base pair repetitive part of the glycoprotein gene. This result was consistent with the results obtained from another orb-weaving spider Nephila clavipes. It is important to know the homologous genes from different species in order to determine the function and evolution of this gene. Furthermore, studying more about the biochemical and molecular properties of the glycoprotein will enable to better understand its role in the aqueous sticky coat as well as make a contribution in the general effort of developing the novel bio-based glue. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Glycoproteins--Synthesis--Research; Araneus--Genetics

Related Item:series Title Information
Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng