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Bacteriophage-mediated Biocontrol of Escherichia ...

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Title Information
Bacteriophage-mediated Biocontrol of Escherichia coli O157:H7 in Soil

Name:Personal
Bolenbaugh, Kyle E. Role :Text(marcrelator)
creator

Name:Personal
Department of Animal Science; John D. Willford Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-23

Language:Text
eng

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born digital

abstract
Escherichia coli O157:H7 continues to be a major foodborne pathogen. Recent outbreaks have occurred in ready to eat (RTE) fruits and vegetables that have been attributed to the presence of E. coli O157:H7 in soil. These recent outbreaks have perpetuated a need for increased control measures against pathogenic bacteria in soil. Bacteriophages, viruses which only infect bacteria, provide a safe, natural option of biocontrol against these pathogens. A cocktail of six bacteriophages specific against E. coli O157:H7 was developed and tested as a practical means for reducing this bacterium in soil. Broth tests using a multiplicity of infection (MOI) of 100 showed the complete elimination of an E. coli O157:H7 culture with a 105 CFU/ml concentration within an hour with no evidence of resistance. Soil samples were inoculated with 106 CFU/mL in water and kept moist until cell counts stabilized. The bacteriophage cocktail was utilized to treat the soil at a MOI of 25. No significant reduction in E. coli O157:H7 was observed in the soil. The complex makeup of the soil likely retards the spread of bacteriophage and renders the control measure less effective. However, the efficacy in broth shows the great potential of bacteriophage as a biocontrol and certainly supports the need for further exploration. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Escherichia coli O157:H7--Biological control; Bacteriophages; Soil remediation--Research

Related Item:series Title Information
Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng