NK3 Receptors in the Paraventricular Nucleus of Rats

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NK3 Receptors in the Paraventricular Nucleus of Rats

Name:Personal
Dunbar, Kevin
Role :Text(marcrelator)
creator

Name:Personal
Neuroscience: Department of Zoology & Physiology; Mr. Dane Jensen
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still image
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Powerpoint/pdf
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2008-04-23

Language:Text
eng

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abstract
The research conducted centers on brain neuropeptides called tachykinins that play a major role in regulating salt intake. This research focused even further on the tachykinin, neurokinin 3 receptor (NK3R), which is a G-protein coupled receptor located in the paraventricular nucleus (PVN) of the hypothalamic region of the brain. The nuclear transport of NK3R was examined in male Charles River rats that were given no treatment or an intragastric load of 2 M NaCl. The rats displaying no treatment were sacrificed after 40 minutes, those given the intragastric load of NaCl were sacrificed immediately. After sacrificing, the brains were removed, the PVN was blocked and the nuclei were fixed using a solution of Para formaldehyde and Gluteraldehyde in PBS. Sections of the brains were cut using an ultra microtome and placed on formvar coated copper grids. Different samples of brain sections were incubated with two different antibodies against different sequences of the NK3R and then a second antibody labeled with gold beads. A small amount of gold beads were present in rats sacrificed immediately, but the density of NK3R gold beads within the nucleus increased 12 fold in the rats that were given the intragastic load of NaCl and sacrificed after 40 minutes. This large increase in NK3R may suggest that NK3R is directly regulated by the genome and affects how PVN neurons respond to osmotic challenges.
note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Biology--Study and teaching (Secondary); Tachykinins--Receptors--Research

Related Item:series Title Information
Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng