Effects of Neonatal Exposure to Progesterone on ...

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Effects of Neonatal Exposure to Progesterone on Testicular Morphology in the Rat

Name:Personal
Linsay Edinger
Role :Text(marcrelator)
creator

Name:Personal
Dr. Brenda Alexander
Role :Text(marcrelator)
creator

typeOfResource
text
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Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

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born digital

abstract
Failure of Sertoli cells to mature has been proven to contribute to disorders of testicular function through inability to express functions supporting spermatogenesis. The objective of this experiment was to determine the effect of neonatal progesterone (P4) or progesterone receptor antagonist (RU486) treatment on testicular morphology. Neonatal male pups received RU486, P4, or vehicle (n = 7/group) postnatally d 1 - 5. Testes were collected, weighed, and preserved for histological evaluation at 10.5 weeks. Growth was similar among treatment groups (P = 0.98), but total testes weight was decreased in RU486 (P = 0.04) but not P4 (P = 0.69) males. Serum concentrations of testosterone were similar (P > 0.05) among control and treated males. Numbers of Sertoli cells were evaluated in paraffin embedded hemotoxilyn/eosin stained sections. Variations in Sertoli cell numbers will be used to compare testicular morphology. Testes morphology was evaluated at 20x magnification in digitized images using Spot Advanced and Image J. A comparison of Sertoli cell numbers between treatment groups will indicate the effect of P4 and RU486 on testicular morphology. Changes in testes weight in absence of differences in serum concentrations of testosterone may be reflected in numbers of Sertoli cell which may affect adult fertility.
note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

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http://hdl.handle.net/10176/wyu:730

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http://digital.uwyo.edu/copyright.htm