Parasitology – Practicing Science in the Secondary ...

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Parasitology – Practicing Science in the Secondary Science Classroom

Name:Personal
Jensen, Stephanie
Role :Text(marcrelator)
creator

Name:Personal
Department of Zoology and Physiology and University of Wyoming/Casper College Center; Dr. Dagmara Motriuk-Smith, and Dr. R. Scott Seville
Role :Text(marcrelator)
contributor

typeOfResource
still image
genre
Powerpoint/pdf
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2008-04-23

Language:Text
eng

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born digital

abstract
The research conducted by the resident teacher involved isolation of DNA from intestinal protozoan parasites in the genus Eimeria (Apicomplexa: Eimeriidae). Fecal samples collected from Wyoming ground squirrels (Spermophilus elegans) and black-tailed prairie dogs (Cynomys ludovicianus) were screened for the presence of Eimeria species. Isolated parasite infective bodies (oocysts) were identified to species based on standard morphological characteristics. Two species, Eimeria callospermophili and Eimeria vilasi, were recovered and identified. Oocysts were isolated and concentrated by a series of washings and flotations. DNA extraction and PCR amplification were conducted and the generated DNA sequences will allow for elucidation of the evolutionary relationships of the recovered Eimeria species. During student teaching the research techniques learned and developed above were brought into a high school biology classroom and used to introduce the concepts of transcription, translation, and recombinant DNA technology. Escherichia coli cells were transformed with the pGLO plasmid containing green fluorescence protein gene (GFP). GFP expression was detected by exposure to UV light. The unit developed was implemented in a high school biology course and included the theoretical background and hands-on activities for 50+ students. The depth of topics covered and data analysis was adjusted depending on grade level of students.
note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Eimeria--Wyoming--Identification; Ground squirrels--Parasites--Wyoming--Identification; Genetics--Study and teaching (Secondary)

Related Item:series Title Information
Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng