Effect Of Growth Conditions On Expression of the ...

Object Details

View

Title Information
Effect Of Growth Conditions On Expression of the Type III Secretion System In Verrucomicrobium spinosum

Name:Personal
Gavin Lawlis
Role :Text(marcrelator)
creator

Name:Personal
Naomi Ward
Role :Text(marcrelator)
creator

typeOfResource
text
genre
Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

Physical Description
born digital

abstract
Type III secretion systems (T3SS), one of six known types of secretion systems in bacteria, are known for their role in secreting toxic proteins into host cells causing disease. Their expression is frequently affected by environmental conditions such as temperature and calcium availability. Genome sequencing and annotation of Verrucomicrobium spinosum, a member of the PVC (Planctomycetes-Verrucomicrobia-Chlamydia superphylum), revealed genes encoding all of the required components of a T3SS. The functions mediated by this novel T3SS are currently under investigation. To better understand how the V. spinosum T3SS is expressed under different conditions, we performed quantitative reverse-transcriptase PCR (qRT-PCR) of selected predicted T3SS genes. We used a range of environmental conditions, including different temperatures, sugar substrates, and NaCl concentrations. Growth curves were constructed via spectrophotometric measurements of cell densities. mRNA was extracted from different culture time points and specific PCR primers were then used to selectively and quantitatively amplify genes encoding different T3SS components. By looking at the different expression levels of the T3SS genes, the data will give insight as to how the T3SS may be regulated under different environmental conditions.
note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

Location (usage="primary display")
http://hdl.handle.net/10176/wyu:729

accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm