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Discovering Crucial Components of the ESRE Network: ...
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Discovering Crucial Components of the ESRE Network: a Novel Stress-Response System
Discovering Crucial Components of the ESRE Network: a Novel Stress-Response System
Name:Personal
Morgan Wichelhaus Role :Text(marcrelator)
creator
Morgan Wichelhaus Role :Text(marcrelator)
creator
Name:Personal
Dr. David Fay Role :Text(marcrelator)
creator
Dr. David Fay Role :Text(marcrelator)
creator
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Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
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born digital
abstract
Pleiotropic response to various environmental stresses distinguishes the ESRE (ethanol and stress response element) stress-response network from other stress systems. Along with this distinction, the degree of evolutionary conservation makes the ESRE network a novel and significant pathway to study. The objective of our research is to 1) identify the transcription factor that binds directly to the conserved ESRE motif (TCTGCGTCTCT) and 2) identify other components of the ESRE network. Efforts to identify the ESRE binding protein, or EBP, are currently underway at the time of abstract submission. Our approach includes C. elegans RNAi screens and yeast one-hybrid screens. Worms carrying a 3xESRE::GFP construct and fed a potential EBP RNAi clone will show reduced fluorescence when compared to unfed controls under stress conditions. In yeast one-hybrid screens, a 3xESRE::lacZ reporter construct will show activation upon binding with a likely EBP transcription factor fused with the gal4 activation domain. Future research will seek to identify other regulatory genes through additional RNAi screens and/or forward genetic screens. The elucidation of the EBP and other ESRE network components will further our understanding of the molecular mechanisms behind stress-response, and may provide targets for new pharmaceuticals and medical assays. note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
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Undergraduate Research Day
Undergraduate Research Day
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Undergraduate Research Day 2010
Undergraduate Research Day 2010
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http://hdl.handle.net/10176/wyu:726
http://hdl.handle.net/10176/wyu:726
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