Discovering Crucial Components of the ESRE Network: ...

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Discovering Crucial Components of the ESRE Network: a Novel Stress-Response System

Name:Personal
Morgan Wichelhaus
Role :Text(marcrelator)
creator

Name:Personal
Dr. David Fay
Role :Text(marcrelator)
creator

typeOfResource
text
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Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

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born digital

abstract
Pleiotropic response to various environmental stresses distinguishes the ESRE (ethanol and stress response element) stress-response network from other stress systems. Along with this distinction, the degree of evolutionary conservation makes the ESRE network a novel and significant pathway to study. The objective of our research is to 1) identify the transcription factor that binds directly to the conserved ESRE motif (TCTGCGTCTCT) and 2) identify other components of the ESRE network. Efforts to identify the ESRE binding protein, or EBP, are currently underway at the time of abstract submission. Our approach includes C. elegans RNAi screens and yeast one-hybrid screens. Worms carrying a 3xESRE::GFP construct and fed a potential EBP RNAi clone will show reduced fluorescence when compared to unfed controls under stress conditions. In yeast one-hybrid screens, a 3xESRE::lacZ reporter construct will show activation upon binding with a likely EBP transcription factor fused with the gal4 activation domain. Future research will seek to identify other regulatory genes through additional RNAi screens and/or forward genetic screens. The elucidation of the EBP and other ESRE network components will further our understanding of the molecular mechanisms behind stress-response, and may provide targets for new pharmaceuticals and medical assays.
note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

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http://hdl.handle.net/10176/wyu:726

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http://digital.uwyo.edu/copyright.htm