Development of a lipid peroxidation assay for ...

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Development of a lipid peroxidation assay for filamentous fungi for cellular stress assessment.

Name:Personal
Berthal J. Devilbiss
Role :Text(marcrelator)
creator

Name:Personal
Joshua Sharpe
Role :Text(marcrelator)
creator

Name:Personal
B.J. Troxell
Role :Text(marcrelator)
creator

Name:Personal
Dr. Ami Wangeline
Role :Text(marcrelator)
creator

typeOfResource
text
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Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

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born digital

abstract
The amount of lipid peroxidation in an organism has been found to be an indicator for cellular stress. In filamentous fungi growth retardation and alteration of normal pigmentation can be used as indicators of cellular stress, however more quantitative methods are desired as well as the ability to detect stress when no morphological alterations are observed. During the process of lipid peroxidation polyunsaturated fatty acids decompose and malondaildehyde (MDA), a secondary end product is produced. One method, the Thiobarbituric acid-reactive substances method (TBARS), in which MDA is reacted with Thiobarbituric acid (TBA) producing a colorimetric product that can be measured spectrophotometrically at 532 nm has been used extensively in plants but has issues with interfering compounds that are also active at 532nm. The purpose of the current study was to evaluate various modifications of the TBARS assay for use in stress evaluation in several genera of filamentous fungi for comparison to other metabolic information such as antioxidant capacity and phenolic content.
note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

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http://hdl.handle.net/10176/wyu:721

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http://digital.uwyo.edu/copyright.htm