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Development of a lipid peroxidation assay for ...
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Development of a lipid peroxidation assay for filamentous fungi for cellular stress assessment.
Development of a lipid peroxidation assay for filamentous fungi for cellular stress assessment.
Name:Personal
Berthal J. Devilbiss Role :Text(marcrelator)
creator
Berthal J. Devilbiss Role :Text(marcrelator)
creator
Name:Personal
Joshua Sharpe Role :Text(marcrelator)
creator
Joshua Sharpe Role :Text(marcrelator)
creator
Name:Personal
B.J. Troxell Role :Text(marcrelator)
creator
B.J. Troxell Role :Text(marcrelator)
creator
Name:Personal
Dr. Ami Wangeline Role :Text(marcrelator)
creator
Dr. Ami Wangeline Role :Text(marcrelator)
creator
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Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
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born digital
abstract
The amount of lipid peroxidation in an organism has been found to be an indicator for cellular stress. In filamentous fungi growth retardation and alteration of normal pigmentation can be used as indicators of cellular stress, however more quantitative methods are desired as well as the ability to detect stress when no morphological alterations are observed. During the process of lipid peroxidation polyunsaturated fatty acids decompose and malondaildehyde (MDA), a secondary end product is produced. One method, the Thiobarbituric acid-reactive substances method (TBARS), in which MDA is reacted with Thiobarbituric acid (TBA) producing a colorimetric product that can be measured spectrophotometrically at 532 nm has been used extensively in plants but has issues with interfering compounds that are also active at 532nm. The purpose of the current study was to evaluate various modifications of the TBARS assay for use in stress evaluation in several genera of filamentous fungi for comparison to other metabolic information such as antioxidant capacity and phenolic content. note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
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Undergraduate Research Day
Undergraduate Research Day
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Undergraduate Research Day 2010
Undergraduate Research Day 2010
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http://hdl.handle.net/10176/wyu:721
http://hdl.handle.net/10176/wyu:721
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