Home › Digital Repository › Faculty, Staff and Student Publications/Presentations › Undergraduate Research › Undergraduate Research Day › Undergraduate Research Day 2010 ›
Cloning, Expression, and Purification of Recombinant ...
Object Details
View
Title Information
Cloning, Expression, and Purification of Recombinant Yersinia enterocolitica 0:9 LcrV and its Application In a Serologic Assay for Yersiniosis in Infected Elk
Cloning, Expression, and Purification of Recombinant Yersinia enterocolitica 0:9 LcrV and its Application In a Serologic Assay for Yersiniosis in Infected Elk
Name:Personal
Whitney Ellsbury Role :Text(marcrelator)
creator
Whitney Ellsbury Role :Text(marcrelator)
creator
Name:Personal
Dr. Gerard Andrews Role :Text(marcrelator)
creator
Dr. Gerard Andrews Role :Text(marcrelator)
creator
typeOfResource
text genre
Powerpoint/PDF
Origin Information
Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
Physical Description
born digital
born digital
abstract
Brucellosisis is a zoonotic disease that causes abortion in domestic and wild ruminants. In particular, the disease persists in free-ranging elk inhabiting the Greater Yellowstone Area. Serologic surveillance in this host is difficult due to cross-reactivity associated with antibody to lipopolysaccharide of Yersinia enterocolitica. Specificity of the current diagnostic assay may thus be improved by employing the use of Yersinia-specific antigens as serologic targets to differentiate between B. abortus and Y. enterocolitica infection. In this regard, previously cloned homologous genes of lcrV and yopD from Y. pestis were expressed in E. coli. The recombinant proteins were purified and immunoblotted against sera from elk experimentally infected with either Y. enterocolitica or B. abortus. Analysis of pooled samples in a slot-blot immune-assay showed a clear delineation in reactivity between animal groups infected with either pathogen. In a Western Blot analysis of individual serum samples, 6/6 animals infected with Yersinia were cross-reactive to Brucella LPS, but all reactive to the Yersinia-specific protein, LcrV, as well. In contrast, four Brucella infected animals were all negative to the Yersinia-specific antigen (p=0.005). We conclude that at least two Yersinia-specific antigens may be usable to delineate between animals infected with either Yersinia or Brucella. note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day
Undergraduate Research Day
Related Item:Host
Title Information
Undergraduate Research Day 2010
Undergraduate Research Day 2010
Location
(usage="primary display")
http://hdl.handle.net/10176/wyu:711
http://hdl.handle.net/10176/wyu:711
accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm