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Caspase-3 activity inhibition in selenophilic ...
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Caspase-3 activity inhibition in selenophilic filamentous fungi
Caspase-3 activity inhibition in selenophilic filamentous fungi
Name:Personal
Hannah Postma Role :Text(marcrelator)
creator
Hannah Postma Role :Text(marcrelator)
creator
Name:Personal
Dan Wu Role :Text(marcrelator)
creator
Dan Wu Role :Text(marcrelator)
creator
Name:Personal
Dr. Ami Wangeline Role :Text(marcrelator)
creator
Dr. Ami Wangeline Role :Text(marcrelator)
creator
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Powerpoint/PDF
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Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
Laramie, Wyoming
University of Wyoming (keyDate="yes")
4/24/2010
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born digital
abstract
Apoptosis is the process of programmed cell death that occurs in organisms. In the cell metabolic process, apoptotic imbalance can lead to many diseases, including cancer and Alzheimer's disease. Caspase-3 is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases and several reports suggest that Caspase-3 is a fundamental component of the apoptotic signaling cascade. Our research used the CASPASE-3 Assay Kit for Drug Discovery (BioMol) to detect inhibition of activity of Caspase-3 by providing a substrate that is cleaved by caspase-3 producing a colorimetrically active product. Inhibition capacity was determined in total extracts from several of genera of selenophilic filamentous fungi cultured with and without Se. Absorbency was recorded over time, and the slopes were analyzed to determine activity while using a provided inhibitor and a non-inhibited control. Extracts from Absidia (zygomycota) and Fusarium (ascomycota) were both found to have high inhibition against the caspase-3 enzyme regardless of growth conditions and extraction method, where as inhibitory impact of other extracts seemed to correlate with extraction method. These inhibitors have the potential to impact apoptotic events when cell death is incorrectly triggered due to disease and we will attempt to elucidate the specific compounds in future studies. note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
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Undergraduate Research Day
Undergraduate Research Day
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Undergraduate Research Day 2010
Undergraduate Research Day 2010
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http://hdl.handle.net/10176/wyu:706
http://hdl.handle.net/10176/wyu:706
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http://digital.uwyo.edu/copyright.htm