Spectroscopic detection of Z-DNA in short ...

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Spectroscopic detection of Z-DNA in short oligonucleotides

Name:Personal
Murtaza Shabbir-Hussain
Role :Text(marcrelator)
creator

Name:Personal
Milan Balaz
Role :Text(marcrelator)
creator

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text
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Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

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born digital

abstract
The structure of right-handed double helical DNA was discovered in 1953 by James D. Watson and Francis Crick. DNA is known to exist in many possible conformations that include A-DNA, B-DNA, C-DNA, D-DNA and Z-DNA. The right-handed B-DNA is the predominant form in biological systems. The B-to-Z-DNA conformation transition is well studied process by which the right-handed duplex turns into a left handed helix known as Z-DNA (zig-zag DNA). Z-DNA exists as a higher energy state of the canonical B-DNA. Recent studies suggest that Z-DNA plays a role in replication and transcription processes. | Our research explored the potential of charged porphyrins as bimolecular recognition tools of short Z-DNA oligonucleotides. We used a short self-complementary oligonucleotide 5 -(dCdG)12-3 for our studies. Selected concentrations of NiCl2 and MgCl2 were used to induce the left-handed Z-DNA form. Following induction, anionic water-soluble tetrasulfonated metalloporphyrins, namely NiTPPS, ZnTPPS, CuTPPS and MnTPPS were used in UV-vis and circular dichroism binding experiments. We will present the spectroscopic recognition of the Z-DNA using the nickel(II) porphyrin.
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From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

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http://digital.uwyo.edu/copyright.htm
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http://hdl.handle.net/10176/wyu:666