Rapid Virulence Annotation Screening of the Putative ...

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Rapid Virulence Annotation Screening of the Putative Pathogen Verrucomicrobium spinosum

Name:Personal
Kristie Capson
Role :Text(marcrelator)
creator

Name:Personal
Naomi L. Ward
Role :Text(marcrelator)
creator

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text
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Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

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born digital

abstract
Verrucomicrobium spinosum is a member of the verrucomicrobia, a bacterial group which is very widespread in the environment, found in eutrophic ponds, lakes, soil habitats and the gastrointestinal tract of humans. Despite this ubiquity, we know very little about the role of verrucomicrobia in these environments. Sequencing of the V. spinosum genome revealed the presence of putative pathogenesis genes, and V. spinosum has also been shown to be pathogenic in animal infection models. However, the connection between the pathogenesis genes and the killing of the animal infection models has not yet been demonstrated. We have used Rapid Virulence Annotation, a functional genomic screen, to determine the specific genes in V spinosum that are toxic to amoebae. V. spinosum genomic DNA was extracted, and used to construct a cosmid library in E.coli. Individual cosmids (1,500) were then screened for gain of toxicity against amoebae. We identified 8 V. spinosum genome regions toxic to the amoebae, and are currently identifying the specific toxic genes within these regions by transposon mutagenesis and re-screening. The results of our work will provide some insight into mechanisms that may be used by V. spinosum to interact pathogenically with a natural host.
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From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
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Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

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http://digital.uwyo.edu/copyright.htm
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http://hdl.handle.net/10176/wyu:627