Simulated RNA Interference in the T4 Bacteriophage ...

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Title Information
Simulated RNA Interference in the T4 Bacteriophage Lytic Cycle

Name:Personal
Jacob Greenlee
Role :Text(marcrelator)
creator

Name:Personal
Dr. John Willford
Role :Text(marcrelator)
creator

typeOfResource
text
genre
Powerpoint/PDF
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
4/24/2010

Physical Description
born digital

abstract
RNA interference pathways have shown promise in disrupting the translation of protein products in eukaryotic cells and organisms. These studies have used siRNA or miRNA, but not mRNA. Bacteria lack the genes to produce the RNAi enzymes found in eukaryotes. In this study, an attempt to simulate an RNAi pathway in hopes of using E. coli as a model organism for mRNA viral therapeutics was made. Part of the T4 K10 bacteriophage T-holin gene (~125kb) has been successfully cloned into the pET expression vector. To disrupt viral mRNA and translation, a complimentary clone needs to be generated and has yet to be accomplished. Initial controls have demonstrated that the presence of the plasmid and pseudogene expression of the partial T-holin mRNA do not interfere with viral replication.
note
From - Undergraduate Research Day 2010 - Celebration of Research - Abstracts
Subject
Undergraduate Research Day

Related Item:Host Title Information
Undergraduate Research Day 2010

accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm
Location (usage="primary display")
http://hdl.handle.net/10176/wyu:623