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Discoveries in Myxococcus xanthus Motility by tgl ...

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Discoveries in Myxococcus xanthus Motility by tgl Mutagenesis

Name:Personal
Woodward, Megan Role :Text(marcrelator)
creator

Name:Personal
Wall, Dr. Daniel Role :Text(marcrelator)
contributor

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still image genre
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Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-18

Language:Text
eng

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born digtal

abstract
Myxobacteria move via gliding motility and are capable of forming ripples, coherent S (Social) motile swarms. Tgl is necessary for the activation of the type IV pili (Tfp), which powers Smotility. When cells are isolated S-motility is absent, suggesting that this motility requires cell-to-cell contact for movement. One reason cell-to-cell interactions of Myxobacteria, more specifically, Myxococcus xanthus are being studied is because their cellular coordinating ability exemplifies a simple multicellular organism. For multicellular motility to be feasible, cells need to communicate for cooperative cell functions. We hypothesize that one way cells can communicate is through cell contact mediated transfer of the Tgl and CglB lipoproteins. In order to study how Tgl might function in cell-cell interactions we sought to create a library of mutants with varying genotypes and phenotypes in order to help elucidate structure-function relationship of Tgl. Error-prone PCR and hydroxylamine mutagenesis were utilized to create a range of tgl mutants. After experimentation, error-prone PCR did not provide a high quality library and the hydroxylamine mutagenesis experimentation finished with inconclusive results and was taken on by a graduate student in the lab. Additional adjustment of procedures should lead to further insight into the role of Tgl in M. xanthus motility. note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Myxococcus xanthus motility

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Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
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English :Code(ISO639-2B)
eng