Differentiation between infections caused by ...

Object Details

View

Title Information
Differentiation between infections caused by Brucella abortus and Yersinia enterocolitica 0:9 in Elk by Western Blot.

Name:Personal
Thompson, Samantha
Role :Text(marcrelator)
creator

Name:Personal
Andrews, Dr Gerard
Role :Text(marcrelator)
contributor

typeOfResource
still image
genre
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-18

Language:Text
eng

Physical Description

born digtal

abstract
Brucellosis is a disease which impacts both domestic and wildlife animal populations. Elk have been misdiagnosed with brucellosis by the standard serologic laboratory assay (ELISA), because of potential cross-reactivity with non-Brucella antigens. This phenomenon is likely due to similarity of the composition of the O-polysaccharide side-chain of lipopolysaccharide (LPS) with Yersinia enterocolitica. Chronic infection by this enteric species in elk may, therefore, confound assay results. To address this problem, we examined LPS from Y. enterocolitica and B. abortus by immunoblot against sera from elk, infected with Y. enterocolitica O:9. LPS probed with sera from Yersinia-infected animals showed equally strong reactivity to o-polysaccharide from both species. We next evaluated the anti-Yersinia and Brucella serum samples against a purified recombinant Yersinia protein, LcrV, with no known homolog in B. abortus. Six of 8 samples from Yersiniainfected elk were positive, while none of the Brucella-POS samples showed specificity for LcrV. Conversely, all 8 anti-Yersinia samples were negative against a protein unique to Brucella, AfuA, while 8 of 9 anti-Brucella samples were positive. We conclude that that cross-reactivity can be abrogated through the use of protein antigens unique to each species. A modified/augmented assay may therefore delineate between yersiniosis, brucellosis, or co-infection.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
elk brucellosis

Subject
elk yersiniosis

Subject
cross-reactivity

Related Item:series Title Information
Undergrauate Research Day 2009

Location (usage="primary display")

accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng