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Differentiation between infections caused by Brucella abortus and Yersinia enterocolitica 0:9 in Elk by Western Blot.
Differentiation between infections caused by Brucella abortus and Yersinia enterocolitica 0:9 in Elk by Western Blot.
Name:Personal
Thompson, Samantha Role :Text(marcrelator)
creator
Thompson, Samantha Role :Text(marcrelator)
creator
Name:Personal
Andrews, Dr Gerard Role :Text(marcrelator)
contributor
Andrews, Dr Gerard Role :Text(marcrelator)
contributor
typeOfResource
still image genre
Origin Information
Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-18
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-18
Language:Text
eng
eng
Physical Description
born digtal
born digtal
abstract
Brucellosis is a disease which impacts both domestic and wildlife animal populations. Elk have been misdiagnosed with brucellosis by the standard serologic laboratory assay (ELISA), because of potential cross-reactivity with non-Brucella antigens. This phenomenon is likely due to similarity of the composition of the O-polysaccharide side-chain of lipopolysaccharide (LPS) with Yersinia enterocolitica. Chronic infection by this enteric species in elk may, therefore, confound assay results. To address this problem, we examined LPS from Y. enterocolitica and B. abortus by immunoblot against sera from elk, infected with Y. enterocolitica O:9. LPS probed with sera from Yersinia-infected animals showed equally strong reactivity to o-polysaccharide from both species. We next evaluated the anti-Yersinia and Brucella serum samples against a purified recombinant Yersinia protein, LcrV, with no known homolog in B. abortus. Six of 8 samples from Yersiniainfected elk were positive, while none of the Brucella-POS samples showed specificity for LcrV. Conversely, all 8 anti-Yersinia samples were negative against a protein unique to Brucella, AfuA, while 8 of 9 anti-Brucella samples were positive. We conclude that that cross-reactivity can be abrogated through the use of protein antigens unique to each species. A modified/augmented assay may therefore delineate between yersiniosis, brucellosis, or co-infection. note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
elk brucellosis
elk brucellosis
Subject
elk yersiniosis
elk yersiniosis
Subject
cross-reactivity
cross-reactivity
Related Item:series
Title Information
Undergrauate Research Day 2009
Undergrauate Research Day 2009
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http://digital.uwyo.edu/copyright.htm
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:Text(ISO639-2B)
English :Code(ISO639-2B)
eng
English :Code(ISO639-2B)
eng