Serologic Characterization of a Potential Virulence ...

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Serologic Characterization of a Potential Virulence Gene Product from Brucella abortus

Name:Personal
Spellman, Jessie
Role :Text(marcrelator)
creator

Name:Personal
Andrews, Gerard
Role :Text(marcrelator)
contributor

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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-18

Language:Text
eng

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born digtal

abstract
Brucella abortus is the causative agent of brucellosis in ungulates. Infection causes spontaneous, premature abortion and can therefore be a serious problem for wildlife populations, as well as the beef and dairy industries. Current diagnostic tests are ELISA-based, which detect the presence of lipopolysaccharide antibodies in serum. These tests must be performed in the laboratory and are therefore labor-intensive and time consuming. Through the application of in vivo-induced antigen technology (IVIAT), ten B. abortus genes were previously identified that are up-regulated during infection in elk. Specifically for this project, we PCR-amplified, cloned, and expressed one such gene, virJ, encoding a component of a type-IV secretion system required for virulence in B. abortus. We subsequently electroblotted and probed the recombinant protein with twenty-one serum samples from elk that previously tested both negative and positive for brucellosis, as well as elk that were immunized with the B. abortus S19 live vaccine. Sixteen confirmed positive and negative cattle serum samples were also tested. Based on preliminary results, we conclude that VirJ is a predictor of natural infection in elk. Analysis of additional samples should confirm if sero-reactivity to this antigen allows differentiation from vaccination, as well as predicts infection in cattle.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Brucella abortus

Subject
ungulate brucellosis

Subject
VirJ sero-reactivity

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng