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Cloning and Expression of Recombinant PrP Protein ...

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Cloning and Expression of Recombinant PrP Protein From the Moose (Alces alces shirasi) Genomic Variant 209 Ile

Name:Personal
Smylie, Sandra Role :Text(marcrelator)
creator

Name:Personal
Jewell, Jean, PhD. Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-18

Language:Text
eng

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born digtal

abstract
Chronic Wasting Disease is a transmissible spongiform encephalopathy (TSE) that affects white-tail deer (Odocoileus virginianus), mule deer (Odocoileus hemionus), and Rocky Mountain elk (Cervus elaphus nelsoni). The Shira’s moose (Alces alces shirasi) is another North American cervid that inhabits the CWD endemic area of Northern Colorado and Southeastern Wyoming. Although rarely documented, the moose is susceptible to develop CWD. The Shira’s moose has two different genetic alleles of the prion protein, but unlike the situation in deer and elk there is no known correlation between CWD susceptibility and genotype. The aim of this project is to clone and express in bacteria the moose prion protein allele that has an isoleucine at codon 209. The purified recombinant prion protein will then be used to obtain and standardize MALDI (matrix-assisted laser desorption ionization) mass spectroscopy spectra to analyze the allelic composition of disease-associated prions in CWD-infected moose. This analysis will complement similar analyses being done on mule deer, white-tail deer, and elk that also contain genetic differences within the prion protein gene. Furthermore, with the use of standardized mass spectroscopy, continued research will provide insight about the relationship of the genetic variability in moose to the susceptibility and process of CWD. note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
cervid chronic wasting disease

Subject
transmissible spongiform encephalopathy

Subject
Shira’s moose

Subject
Alces alces shirasi

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Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
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English :Code(ISO639-2B)
eng