Mechanistic Studies of Pyrolysis Induced ...

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Mechanistic Studies of Pyrolysis Induced Site-Specific Cleavage at Aspartic Acid by Tandem Mass Spectrometry

Name:Personal
Schunter, Alissa
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Name:Personal
Basile, Dr. Franco
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Laramie, Wyoming

University of Wyoming
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2009-05-18

Language:Text
eng

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born digtal

abstract
Our group’s previous ESI MS-MS studies of peptides indicate that pyrolysis at 220°C for 10 seconds causes a site-specific cleavage at the C-terminus of aspartic acid (D) residues that is accompanied by a loss of water on the C-terminal fragment. Elucidating the mechanism for this cleavage will allow us to use pyrolysis to determine the sequence of unknown peptides. The literature indicates that peptides cyclize during analysis and subsequently re-open at multiple sites around the ring, causing the amino acid sequence to be scrambled, so the first step in elucidating the mechanism for the cleavage at D is to acetylate the N-terminus of the sample peptides to prevent this cyclization. As of the writing of this abstract, experimentation with various methods of acetylation is being carried out. Once satisfactory acetylation is achieved, one peptide sample will be pyrolyzed and acetylated, and its loss of water product ion analyzed by MS-MS. Another sample will also be acetylated and its loss of water fragments analyzed by MS3 without pyrolysis. Since the mechanism for the loss of water under these conditions is well-documented, I expect to deduce the mechanism resulting from pyrolysis by comparing the MS2 spectrum to the MS3 spectrum.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
peptide pyrolisis

Subject
peptide acetylation

Subject
C-terminus cleavage

Related Item:series Title Information
Undergrauate Research Day 2009

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eng