Determination of Phylogenetic Relationships of Three ...

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Determination of Phylogenetic Relationships of Three Eimeria (Apicomplexia: Eimeridae) Species Using 18S-ITS1-5.8S-ITS2-23S rDNA Sequence.

Name:Personal
Quealy, Leah
Role :Text(marcrelator)
creator

Name:Personal
Motriuk-Smith, Dagmara
Role :Text(marcrelator)
contributor

Name:Personal
Seville, R. Scott
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contributor

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still image
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Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-15

Language:Text
eng

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born digtal

abstract
The taxonomy and classification of parasites in the genus Eimeria (Apicomplexa: Eimeriidae) has been based on oocyst morphology, host specificity and host geographic distribution. However, identification of species sharing similar morphologies or displaying broad host ranges is difficult based on these characteristics alone. In this study, we compare sequence data from the ITS1 and ITS2 rDNA regions of three Eimeria species collected from six species of wild rodent hosts to determine if they provide additional characteristics useful in resolving taxonomic difficulties. ITS1 and ITS2 rDNA sequences were obtained from Eimeria callospermophili isolated from Wyoming ground squirrels (Spermophilus elegans), yellow bellied marmots (Marmota flaviventris), white tailed prairie dogs (Cynomys leucurus), and black tailed prairie dogs (Cynomys ludovicianus), Eimeria lancasterensis from Wyoming fox squirrels (Sciuris niger) and Virginia Delmarva fox squirrels (Sciurus niger cinereus), and Eimeria ontarioensis from Wyoming fox squirrels. Phylogenetic hypotheses generated using maximum parsimony and neighbor joining analysis of the ITS1 and ITS2 regions supported the conspecificity of the four E. callospermophili isolates. Additionally, E. lancasterensis and E. ontarioensis formed two distinct monophyletic lineages though the WY and VA strains of E. lancasterensis were unable to be resolved.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Apicomplexa: Eimeriidae

Subject
taxonomy

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng