Piriform PCR: Searching for Spider Silk Sequences

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Piriform PCR: Searching for Spider Silk Sequences

Name:Personal
Neuwerth, Thomas
Role :Text(marcrelator)
creator

Name:Personal
Lewis, Dr. Randy
Role :Text(marcrelator)
contributor

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still image
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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-15

Language:Text
eng

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born digtal

abstract
Our research goal was the isolation of the gene encoding piriform silk from Araneus gemmoides. This protein, expressed by the piriform silk gland, acts as the “cement” between joints in a spider’s web. Of the six different types of silks produced by orb-weaving spiders, piriform silk proteins were the single silk type for which there was no sequence data. Piriform cDNA sequences have been recently isolated in our laboratory for three other spider species, but not for A. gemmoides. Polymerase Chain Reaction (PCR) primers were designed from the conserved 3’-regions of the known piriform sequences and were used to amplify genomic DNA from A. gemmoides. The hypothesis was that consensus and degenerate primers from the three known piriform sequences would have sufficient sequence homology to the A. gemmoides gene to provide a basis for PCR amplification. Genomic DNA was isolated from spiders captured in the Laramie area. PCR products were analyzed by agarose gel electrophoresis; presence of a band indicated a successful amplification. After a successful amplification PCR fragments were excised from gels, eluted, and submitted for sequencing. At this point one sequence has been produced; research is continuing and should produce additional sequences in the future.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
piriform silk

Subject
Araneus

Subject
MaSp1

Subject
recombinant dragline silk protein

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
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English
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eng