Monoclonal Antibody Production Against Synthetic (PrPc)

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Title Information
Monoclonal Antibody Production Against Synthetic (PrPc)

Name:Personal
McDaniel, Kaitlin
Role :Text(marcrelator)
creator

Name:Personal
Belden, Everett Lee, Ph.D.
Role :Text(marcrelator)
contributor

typeOfResource
still image
genre
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-15

Language:Text
eng

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born digtal

abstract
This study investigates the production of monoclonal antibodies against the two different mule deer prion protein allotypes. We hypothesized that antibodies can be made against synthetic prion peptide (PrPc) sequence differences specific for amino acid dimorphisms at position 225. Monoclonal antibodies were produced against synthetic peptide sequences representing (PrPc) 225 F (phenylalanine at position 225) and 225S (serine at position 225) prion peptides and screened by ELISA for reactivity to synthetic (PrPc). Western blots and immunohistochemical analysis will be run to confirm antibody specificity and reactivity. This furthers research on prion biology by allowing genotype determination of tissues and the examination of cellular expression differences that may occur between the two mule deer genotypes.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
monoclonal antibodies

Subject
mule deer

Subject
prion protein allotypes

Subject
synthetic prion peptide (PrPc)

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng