SERS Detection of Viral DNA using Morpholino Oligos ...

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SERS Detection of Viral DNA using Morpholino Oligos Tethered to Colloidal Gold Nanoparticles

Name:Personal
McConnell, Joshua T.
Role :Text(marcrelator)
creator

Name:Personal
Johnson, Patrick, Ph.D
Role :Text(marcrelator)
contributor

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still image
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Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-15

Language:Text
eng

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born digtal

abstract
This study will examine Au nanoparticle based DNA biosensors utilizing morpholino oligos as tethering and signaling probes. Thiol-functionalized and non-functionalized morpholinos are to be obtained from Gene Tools, LLC. The non-functionalized morpholinos (signaling probes) will be modified with a Raman dye. The thiol-fuctionalized morpholinos (tethering probes) will be attached to the nanoparticle surface. Conjugated Au nanoparticles and signaling probes are to be exposed to a sequence of DNA; one being the target West Nile Virus DNA and the other being a control. Both will be analyzed using SERS spectroscopy. Analysis will be performed with buffers of differing strengths. The results of this experiment will be compared to results obtained from using DNA as the signaling and tethering probes. I hypothesize that an enhanced signal will be observed as a result of using morpholinos in this method of DNA sensing and that the elimination of noise will be observed when using buffers of lower ionic concentration. If the hypothesis is supported, an enhanced method of DNA detection will be available.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Au nanoparticle-based DNA biosensors

Subject
West Nile Virus

Subject
morpholino oligos

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng