Visualization of the Verrucomicrobium spinosum type ...

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Visualization of the Verrucomicrobium spinosum type III secretion system needle protein using Transmission electron microscopy

Name:Personal
McCann, Sage
Role :Text(marcrelator)
creator

Name:Personal
Sait, Dr. Michelle
Role :Text(marcrelator)
contributor

Name:Personal
Ward, Dr. Naomi
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contributor

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still image
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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-15

Language:Text
eng

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born digtal

abstract
Genome sequencing of Verrucomicrobium spinosum, the type strain of the phylum Verrucomicrobia, has revealed the presence of a Type III secretion system (T3SS). The presence of a T3SS is typically indicative of a pathogenic interaction with a eukaryotic cell; however, no pathogenic or symbiotic interactions have been reported in V. spinosum. A key component to the T3SS injectisome is the presence of a needle protein that mediates an interaction between the bacterial and eukaryotic cell. We have identified two putative needle proteins in the V. spinosum genome. Our aims are: 1) to determine if the structural components of the T3SS injectisome are being expressed, 2) to visualize the T3SS using transmission electron microscopy (TEM) and 3) to use immunoelectronmicroscopy to identify and localize the putative needle protein. We have successfully extracted RNA from V. spinosum grown under standard conditions. We then PCR amplified the VspC and VspQ T3SS structural proteins, verifying that they are expressed. We have also developed methods to embed, generate thin-sections and visualize V. spinosum using TEM.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Verrucomicrobium spinosum

Subject
Type III secretion system

Subject
T3SS injectisome

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng