Silk Genes and Proteins from Orb-weaving Spiders ...

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Title Information
Silk Genes and Proteins from Orb-weaving Spiders (family Araneidae)

Name:Personal
Keating, Traci J.
Role :Text(marcrelator)
creator

Name:Personal
Motriuk-Smith, Dagmara
Role :Text(marcrelator)
contributor

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still image
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Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-14

Language:Text
eng

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born digtal

abstract
Scientists have been studying spider silk and attempting to produce artificial fibers which could find use in a variety of commercial and medical applications. Although much knowledge about silk has been already acquired, most attempts to recreate a quality silk fiber have not yielded satisfactory results. The purpose of this research project was to identify silk glands and prepare a quality genomic DNA. Fresh tissue samples were obtained from live Argiope trifasciata orbweaving spiders. The spiders were anesthetized and dissected. Major ampullate, minor ampullate, and tubular silk glands were identified based on size, morphology, pigmentation along with the ducts and spigots used in silk secretion. After examination of the glands using a dissecting microscope (100X), the glands were stored for further protein and RNA studies. Genomic DNA was extracted from Latrodectus, Nephila, Tetragnatha, Araneus and Argiope. Fresh and frozen tissue samples were utilized. DNA results from the fresh and frozen tissue samples were compared. Fresh tissue samples yielded the best DNA results as determined by gel electrophoresis and UV spectroscopy. Future experiments will include amplification and sequencing of silk genes.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
spider silk

Subject
artificial fibers

Subject
MaSp1

Subject
recombinant dragline silk protein

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng