Determination of Cell Type and Subcellular Location ...

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Determination of Cell Type and Subcellular Location of Spermidine Synthase

Name:Personal
Grauberger, Kevin
Role :Text(marcrelator)
creator

Name:Personal
Stayton, Dr. Mark
Role :Text(marcrelator)
contributor

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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-13

Language:Text
eng

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abstract
Heart disease is the leading cause of death in the United States. In an effort to understand the early responses in the heart to an acute myocardial infarction (AMI), a microarray study was performed and the arginine-nitric oxide-polyamine pathway was highly up regulated. Immunohistochemistry was used to determine the cell type and subcellular location of spermidine synthase (SPDS), spermine/spermidine acetyl transferase (SSAT), and polyamine oxidase (PAO). Initial immunohistochemistry results for SPDS suggest the enzyme is located in the intercalated discs of cardiomyocytes. Immunohistochemistry for PAO suggests the enzyme is located in fibroblasts. Immunohistochemistry for SSAT did not give conclusive results. Additionally, the researchers attempted to clone a bovine Arginase 1 (ARG1) gene into E. coli for the purpose of overproduction and the gene was successfully introduced into E. coli. Also, increased L-arginine levels in the blood may help increase the chances of surviving an AMI and citruline is a precursor for L-arginine. Blood plasma samples were prepared for amino acid analysis in an attempt to determine if mice with increased dietary citruline levels helped to elevate blood arginine levels.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Heart disease

Subject
Immunohistochemistry

Subject
acute myocardial infarction

Subject
spermidine synthase

Subject
bovine Arginase 1

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
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English
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eng