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Comparison of methods and techniques of DNA ...

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Comparison of methods and techniques of DNA extraction and amplification: Development of reusable learning objects as a biotechnology teaching tool.

Name:Personal
George, Jami Role :Text(marcrelator)
creator

Name:Personal
Treeby, Cameron Role :Text(marcrelator)
creator

Name:Personal
Stoner, Clay Role :Text(marcrelator)
creator

Name:Personal
Dube, Rachele Role :Text(marcrelator)
creator

Name:Personal
Erickson, Ami Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-13

Language:Text
eng

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born digtal

abstract
The manipulation of molecular DNA and genetic engineering are important tools in biotechnology that can positively affect all areas of production in agriculture, food science, and medicine. Multiple protocols for extracting, isolating, and amplifying genetic DNA were explored. Spectrophotometry, gel electrophoresis, and polymerase chain reaction (PCR) were utilized in two DNA extraction and amplification methods in the attempt to yield testable DNA from human hair follicles and plant Spinacia oleracea leaves. Flow charts comparing and contrasting DNA extraction and amplification methods were developed from the procedures as a reusable learning object. The human DNA extraction that was amplified using a primer for the PV92 allele yielded successful results. The methods used for Spinacia oleracea yielded extraction of DNA, but amplification was unsuccessful using the OPAB 07 and 09 primer. Further investigation is needed to determine the specific causes for S. oleracea results. Lab techniques were developed, including proper use of lab equipment such as micropipettes and gel electrophoresis, as well as solution preparation for lab protocols. note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
genetic engineering

Subject
molecular DNA

Subject
DNA extraction techniqies

Subject
biotechnology teaching tools

Related Item:series Title Information
Undergrauate Research Day 2009

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accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng