Isolation and Characterization of Antibiotics from ...

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Isolation and Characterization of Antibiotics from Streptomyces Isolated from Soil Samples

Name:Personal
Fall, Katie
Role :Text(marcrelator)
creator

Name:Personal
Erickson, Ami
Role :Text(marcrelator)
creator

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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-13

Language:Text
eng

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born digtal

abstract
The objective of this study is to identify, isolate and characterize antibiotics produced by three samples of Streptomyces by using antibiotic assays and chromatography. Three samples of Streptomyces were identified as able to produce antibiotics against (Bacillus subtillus, Escherichia coli (E. coli), and Sarcina aurantiaca). By cutting zones of inhibition from agar plates and filtering broth cultures, chromatography will be used to analyze the sample complexity, substance identification, relative composition, and kinetic characteristics of the sample. Streptomyces Sample 1 had excreted light pink pigments into the agar. The zones of inhibition averages for Sample 1 were S.aurantiaca: 2.13 cm, E. coli: 1.30 cm, and B.subtillus: 1.23 cm. Streptomyces Sample 2 was a light yellowish color with no excretion of pigments into the agar. Sample 2 averages were S. aurantiaca: 0.20 cm, E. coli: 0.10 cm, and B. subtillus: 0.40 cm. Streptomyces Sample 3 was a light yellow color with crusty properties. Sample 3 averages were S.aurantiaca: 1.35 cm, E.coli: 0.10 cm, and B.subtillus: 0.67 cm.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
antibiotics

Subject
Streptomyces

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng