Eimeria Identification and Evolutionary ...

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Eimeria Identification and Evolutionary Relationships in the Wyoming Ground Squirrel (Spermophilus elegans)

Name:Personal
Costello, Erica
Role :Text(marcrelator)
creator

Name:Personal
Motriuk-Smith, Dagmara
Role :Text(marcrelator)
creator

Name:Personal
Seville, R. Scott
Role :Text(marcrelator)
creator

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Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-13

Language:Text
eng

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born digtal

abstract
The objective of this study was to identify species of Eimeria occurring in Wyoming ground squirrels (Spermophilus elegans) in central Wyoming. Fecal samples were collected from ground squirrels during the summer of 2008. The animals were live-trapped, their fecal samples were collected and the animals were released. The samples were mixed with 2% potassium dichromate and held at ambient temperature for 7+ days to allow sporulation of Eimeria oocysts. Following sporulation oocysts were isolated from feces by sugar flotation and examined at 100-1000X light microscopy for the presence/absence of Eimeria. Thirty samples were screened and 23 were positive for Eimeria (prevalence = 77%) and 5 species recovered: Eimeria lateralis, Eimeria callospermophili, Eimeria vilasi, and Eimeria cynomysis. Eimeria lateralis positive samples possessing a minimum of 1,000 oocysts were concentrated by repeated sugar floatation and centrifugation. In the future, genomic DNA will be isolated and polymerase chain reaction (PCR) and agarose gel electrophoresis will be performed. 18S rDNA will be sequenced and compared to the other Eimerians in the database.
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From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Wyoming ground squirrels (Spermophilus elegans)

Subject
Eimeria species

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng