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Bacteriophage-mediated Biocontrol of Escherichia ...

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Bacteriophage-mediated Biocontrol of Escherichia coli O157:H7 in Milk and Juice

Name:Personal
Bolenbaugh, K. Edward Role :Text(marcrelator)
creator

Name:Personal
Willford, John D. Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2009-05-13

Language:Text
eng

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born digtal

abstract
Escherichia coli O157:H7 continues to be a major foodborne pathogen. Along with commonly associated foods like beef and spinach, milk and fruit juices have been implicated in numerous outbreaks of E. coli O157:H7 in the United States, Canada and the United Kingdom. Bacteriophages provide a safe, natural option of biocontrol against these bacterial pathogens. A cocktail of six bacteriophages specific for E. coli O157:H7 was developed and tested as a practical means for reducing this bacterium in milk, apple juice, and orange juice. Broth controls using a multiplicity of infection (MOI) of 100 showed the complete elimination of a 105 CFU/mL E. coli O157:H7 culture in less than one hour with no evidence of bacterial resistance. No significant reduction was identified in the apple juice or orange juice trials. However, in a notable milk trial, a 3- log reduction of E. coli O157:H7 was seen. The reductions seen in milk trials indicate that our phage cocktail is viable as a biocrontrol agent. This cocktail could be a safe, economical and effective means to mitigate temperature abuse of milk in commercial industry as well as a potential method of pasteurization in raw liquid consumables. note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Escherichia coli O157:H7

Subject
Bacteriophage control methods

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng