Effects of Aflatoxin Exposure on Male ...

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Effects of Aflatoxin Exposure on Male Reproduction-Project Summary

Name:Personal
Austin, Kathy
Role :Text(marcrelator)
creator

Name:Personal
Kaiser, Amanda
Role :Text(marcrelator)
creator

Name:Personal
Cammack, Kristi
Role :Text(marcrelator)
creator

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still image
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Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-12

Language:Text
eng

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born digtal

abstract
Aflatoxicosis occurs in livestock consuming aflatoxin (AFB1)-contaminated grain. Research has shown that genetic factors affect susceptibility of reproductive organs to aflatoxicosis. Our objectives were to 1) identify males more fertile (i.e. tolerant) and less fertile (i.e. intolerant) after AFB1 treatment through mating tests and 2) identify differentially expressed genes associated with AFB1 tolerance. AFB1 treatment did not affect sperm counts. Fetuses sired by control or AFB1 treated males were counted and examined for abnormalities. AFB1 tolerant and intolerant males were identified based on mean fetuses and mean TUNEL positive testicular cells. Tolerant males produced similar fetus numbers (12.5) as control males (13.4), but a higher (P = 0.01) number than intolerant males (7.6). Intolerant males had numerically greater TUNEL positive cells (136.5) than tolerant (55.0) and control (54.3) males. Microarray analyses were performed on control (n = 3), tolerant (n = 3) and intolerant (n = 3) males. Real-time RT-PCR confirmed numerical upregulation of IBSP and CCK genes and numerical downregulation of PGA, Crisp, and AR genes in AFB1 treated males compared to control males. Similar expression patterns of these genes were observed between tolerant and intolerant males. These results suggest genetic selection for AFB1 tolerance is possible.
note
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
Subject
Aflatoxicosis

Subject
genetic selection for AFB1 tolerance

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
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eng