Proteomic Analysis of Methanogens

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Title Information
Proteomic Analysis of Methanogens

Name:Personal
Allhusen, John
Role :Text(marcrelator)
creator

Name:Personal
Basile, Dr. Franco
Role :Text(marcrelator)
contributor

typeOfResource
still image
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Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2009-05-12

Language:Text
eng

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born digtal

abstract
Due to the ever increasing energy needs of our nation alternative fuel sources are being investigated to fulfill these needs. One type of fuel that could help offset the dependence on fossil fuels is methane. Methanogens are a strain of bacteria that produce methane as a bi-product of normal cellular respiration. To increase the efficiency of methane production, the proteins of these bacteria need to be identified. Escherichia coli bacteria were used as a template organism to test a rapid microwave cell lysis and peptide identification method. Scanning electron microscope (SEM) images provided evidence of cell lysis and matrix-assisted laser desorption ionization (MALDI) provided mass data of the lysed products. This method was then used to analyze the protein products of the lysed methanogen bacteria. The SEM images of the methanogens provided qualitative data that the cells were lysed by the microwave technique. However the mass spectral data obtained using MALDI was inconclusive. The images of the lysed E. coli bacteria provided very strong evidence of cell lysing. The MALDI data supported the images in that peptides were obtained through this method of lysing.
note
Subject
Methanogens

Subject
Escherichia coli

Subject
alternative fuel sources

Related Item:series Title Information
Undergrauate Research Day 2009

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http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng