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Exploring Methods for Determining Gut Microbial ...

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Exploring Methods for Determining Gut Microbial Composition in Crohn’s Patients

Name:Personal
McCann, Sage Role :Text(marcrelator)
creator

Name:Personal
Dr. Naomi Ward, Molecular Biology, University of Wyoming Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-26

Language:Text
Eng

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abstract
Crohn’s is an inflammatory bowl disease that predominately affects the lower intestine; research suggests that it is caused by genetic defects in the autoimmune response. It is not known whether exposure to specific pathogens, or if genetic predisposition alone in the presence of normal gut flora leads to Crohn’s symptoms. A key component to answering this question is the comparison of enteric microbial composition of healthy individuals versus that of Crohn’s patients. Methods being evaluated for the determination of enteric microbial composition are: RFLP/ T-RFLP analysis, sequencing, and micro array phylotyping. Restriction digests have been performed and analyzed using the BioRad Experion system as well as fluorescent T-RFLP; we are also waiting for the first phylochip data to come back. The next step will be the construction of clone libraries and sequencing. It is too early in the research to determine the superiority of one method to another. Sequencing from clone libraries will give the most data; however, it is costly and time consuming – not suitable for clinical applications. An effective RFLP or micro array would be faster; the question is whether or not either application is sensitive enough to be used as a diagnostic tool. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
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Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng