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Cardiac Toll-Like Receptor 4-deficiency Limits ...

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Cardiac Toll-Like Receptor 4-deficiency Limits Ischemic Injury via Up-regulation of AMPK and Down-regulation of p38 MAPK

Name:Personal
Li, Jing Role :Text(marcrelator)
creator

Name:Personal
Dr. Ji Li’s Lab., School of Pharmacy Role :Text(marcrelator)
contributor

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still image genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-26

Language:Text
Eng

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born digital

abstract
BACKGROUND: Toll-like receptor 4 (TLR4) is expressed in the heart and vasculature, when deficient, has been shown to protect the heart against ischemia/reperfusion injury. But the molecular mechanisms of the cardioprotection against ischemic damage of TLR4-deficiency remain unclear. AIM: This study determined whether the cardioprotective effects of TLR4-deficiency may function via activation of AMP-activated protein kinase (AMPK) and/or mitogen-activated protein kinase (MAPK) signaling pathway. METHODS: In vivo regional ischemia was induced by occluding the left anterior descending (LAD) coronary artery in wild type (WT) C3H/HeN and TLR4-deficient C3H/HeJ mice. Immunoblotting analysis was performed to measure the activation of AMPK and TLR4 downstream, p38 MAPK. RESULTS: Intriguingly, the TLR4-deficient C3H/HeJ hearts demonstrated impaired AMPK and p38 MAPK activation compared to WT C3H/HeN hearts, i.e. activation of p38 MAPK (Thr180/Tyr182 phosphorylation) were blunted and activation of AMPK (Thr172 phosphorylation) were augmented during ischemia in C3H/HeJ hearts vs. C3H/HeN hearts (p<0.01, n=6 each). Moreover, the AMPK downstream acetyl CoA carboxylase (ACC) Ser79 phosphorylation induced by ischemia was augmented in C3H/HeJ hearts as well compared to C3H/HeN hearts (p<0.01, n=6 each). CONCLUSION: TLR4-deficiency blunted downstream inflammatory signaling like p38 MAPK pathway, and augmented cardiac AMPK signaling pathway to protect against ischemic injury. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
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Undergrauate Research Day 2008

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Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng