Growth and Purification of Recombinant Spider Silks

Object Details

View

Title Information
Growth and Purification of Recombinant Spider Silks

Name:Personal
Basden, Michael
Role :Text(marcrelator)
creator

Name:Personal
Department of Chemical and Petroleum Engineering; Dr. Patrick Johnson
Role :Text(marcrelator)
contributor

typeOfResource
still image
genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming

University of Wyoming
(keyDate="yes")
2008-04-26

Language:Text
eng

Physical Description

born digital

abstract
Spider silk has many unique physical and chemical properties, which make it suitable for a wide range of applications as a biomaterial for implantable devices. It has superior energy absorbance, elongation, and tensile strength. These traits, combined with the natural biocompatibility and biodegradability of spider silk, make it a promising material for potential tissue engineering applications. However, it is not practical to produce spider silk naturally in any meaningful quantity for biomedical applications, it is necessary to use recombinant spider silk proteins produced from bacterial expression systems. This project has focused on the growth and purification of the proteins produced by the recombinant bacteria. The bacteria were grown via shaker flask fermentation. The bacteria were allowed to grow until the culture reaches an optical density of 0.8, or about five hours. At this point, IPTG was added to induce protein production. The spider silk proteins yielded from fermentation were then purified via liquid chromatography on an AKTA purifier. Excess salts were removed from the protein solution through dialysis. Upon production of sufficient quantities of synthetic spider silk, we will next explore mammalian cell responses to spider silk films.
note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Recombinant proteins--Synthesis; Tissue engineering--Research

Related Item:series Title Information
Undergrauate Research Day 2008

Location (usage="primary display")

accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.htm
Record Information languageOfCataloging :Text(ISO639-2B)
English
:Code(ISO639-2B)
eng