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Characterizing fshr-1 Suppressor Mutants Using ...

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Characterizing fshr-1 Suppressor Mutants Using Genetic Mapping and RNAi Tests

Name:Personal
Anderson, Carly-Ann Role :Text(marcrelator)
creator

Name:Personal
Molecular Biology; Dr. David Fay Role :Text(marcrelator)
contributor

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Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-23

Language:Text
eng

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abstract
Hormones play essential roles in human biology by controlling growth, development, and reproduction. Receptors for the two human gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), as well as thyroid stimulating hormone (TSH) have a single ortholog called FSHR-1 in the nematode C. elegans. The C. elegans fshr-1 gene is non-essential and null mutants show no phenotype. The gene, however, appears to be involved in many biological functions as evidenced by synthetic genetic interactions. When fshr-1 and certain other genes are simultaneously knocked down, a phenotype results. A genome-wide RNAi screen revealed 114 genes that act synthetically with fshr-1 to produce several phenotypes. One synthetic interaction of fshr-1 occurs with the fbf-1 and fbf-2 genes to produce a masculinized phenotype, where hermaphrodites produce excess sperm at the expense of oocytes. This observation loosely connects fshr-1 with the sex determination pathway. In attempts to understand how fshr-1 and fbf genes work together, the Fay lab performed a series of genetic suppressor screens. Suppressed mutants are fertile on fbf(RNAi) plates due to either gain- or loss-of-function alleles in regulatory genes, allowing nematodes to behave as though the fshr-1 pathway or the fbf genes were functional. These mutations are currently being mapped and tested to see whether they suppress other fshr-1 synthetic phenotypes detected in previous screens and to determine where they may act in the fshr-1 pathway. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
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Undergrauate Research Day 2008

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http://digital.uwyo.edu/copyright.htm
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English :Code(ISO639-2B)
eng