Home › Digital Repository › Faculty, Staff and Student Publications/Presentations › Undergraduate Research › Undergraduate Research Day › Undergraduate Research Day 2008 ›

Ancestral Sequence Reconstruction and Homology ...

Object Details

View

Title Information
Ancestral Sequence Reconstruction and Homology Modeling of Deoxyribonucleoside Kinase (dNK)

Name:Personal
Thompson, Chandra Role :Text(marcrelator)
creator

Name:Personal
Molecular Biology; Dr. David Liberles Role :Text(marcrelator)
contributor

typeOfResource
still image genre
Powerpoint/Pdf
Origin Information Place
Laramie, Wyoming
University of Wyoming (keyDate="yes")
2008-04-06

Language:Text
eng

Physical Description

born digital

abstract
Gene duplications are important in the evolution of genes and genomes because with gene duplication, proteins can evolve new functions. The dNK enzyme catalyzes the phosphorylation of dNs to dNMPs in the salvage pathway of deoxyribonucleosides essential for obtaining DNA precursors. Chordates have four forms of the enzyme TK2, dCK, and dGK specific for certain substrates. Arthropods have only one form of the enzyme dNK that can bind and phosphorylate all substrates. Based on phylogenetic analysis it appears that the dNK ancestor of chordates and arthropods is more similar to TK2-like enzymes than dNK. The hypothesis is that the dNK ancestor had greater substrate specificity and through neofunctionalization coupled to gene loss events dNK evolved broader specificity along the insect lineage. Ancestral sequences for dNK were created using ideal topologies from the Ecdysozoa and Coelomata hypotheses of species relationships. The sequences were then modeled to give a 3D structure. The energies for binding sites and the overall enzyme were analyzed for each hypothesis and other dNKs. The analysis of these energies indicates that Ecdysozoa is a more thermodynamically stable model, more likely phylogenetically, and that Coelomata and Ecdysozoa may have binding specificity that is more similar to TK2 than dNK. note
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
Subject
Evolutionary genetics--Research; Phosophotransferases; Drosophila melanogaster--Genetics

Related Item:series Title Information
Undergrauate Research Day 2008

Location (usage="primary display")

accessCondition:useAndReproduction
http://digital.uwyo.edu/copyright.html
Record Information languageOfCataloging :Text(ISO639-2B)
English :Code(ISO639-2B)
eng